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human microglia primary cell culture complete media with serum  (Celprogen Inc)


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    Celprogen Inc human microglia primary cell culture complete media with serum
    Human Microglia Primary Cell Culture Complete Media With Serum, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human microglia primary cell culture complete media with serum/product/Celprogen Inc
    Average 92 stars, based on 8 article reviews
    human microglia primary cell culture complete media with serum - by Bioz Stars, 2026-02
    92/100 stars

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    Image Search Results


    Microglial suppression of RCS rats mediated by co-transplantation of RPCs with M-sEVs. (A) Representative confocal images of Iba1 staining in the RPCs and RPCs + M-sEVs groups at 2 and 4 weeks post transplantation. Enlarged orthogonal view of M-sEVs at the injected area (right panel) shows co-localization of the M-sEVs with microglia in the subretinal space. (B) Statistical analysis of the number of Iba1-positive cells in each group in A. (C, D) Representative confocal images showing the localization of MSC-sEVs (PKH26 staining) with F-actin-labeled BV2 (C) and HMC3 (D) microglial cells stimulated with LPS. Scale bars: 20 μm (A), 10 μm (A, enlarged orthogonal view), 50 μm (C, D). (E) RT-PCR analysis showing relative mRNA expression levels of IL-1β, IL-6, and TNF-α in the mouse (BV2) and human (HMC3) microglial cell lines. Data are expressed as means ± SD ( n ≥ 3 for Iba1 staining; n = 3 for real-time PCR); * P < 0.05, ** P < 0.01, *** P < 0.001 (B: Welch’s t -test; D: Welch’s one-way analysis of variance followed by Dunnett’s T3 multiple-comparison test). DAPI: 4′,6-Diamidino-2-phenylindole; EGFP: enhanced green fluorescent protein; Iba1: ionized calcium-binding adapter molecule 1; IL-1β; interleukin 1 beta; IL-6: interleukin 6; LPS: lipopolysaccharide; M-sEVs: mesenchymal stem cell–small extracellular vesicles; RT-PCR: real-time polymerase chain reaction; RPCs: retinal progenitor cells; RCS: Royal College of Surgeons; TNF-α: tumor necrosis factor α; w: weeks.

    Journal: Neural Regeneration Research

    Article Title: Mesenchymal stem cell–derived small extracellular vesicles enhance the therapeutic effect of retinal progenitor cells in retinal degenerative disease rats

    doi: 10.4103/NRR.NRR-D-23-02108

    Figure Lengend Snippet: Microglial suppression of RCS rats mediated by co-transplantation of RPCs with M-sEVs. (A) Representative confocal images of Iba1 staining in the RPCs and RPCs + M-sEVs groups at 2 and 4 weeks post transplantation. Enlarged orthogonal view of M-sEVs at the injected area (right panel) shows co-localization of the M-sEVs with microglia in the subretinal space. (B) Statistical analysis of the number of Iba1-positive cells in each group in A. (C, D) Representative confocal images showing the localization of MSC-sEVs (PKH26 staining) with F-actin-labeled BV2 (C) and HMC3 (D) microglial cells stimulated with LPS. Scale bars: 20 μm (A), 10 μm (A, enlarged orthogonal view), 50 μm (C, D). (E) RT-PCR analysis showing relative mRNA expression levels of IL-1β, IL-6, and TNF-α in the mouse (BV2) and human (HMC3) microglial cell lines. Data are expressed as means ± SD ( n ≥ 3 for Iba1 staining; n = 3 for real-time PCR); * P < 0.05, ** P < 0.01, *** P < 0.001 (B: Welch’s t -test; D: Welch’s one-way analysis of variance followed by Dunnett’s T3 multiple-comparison test). DAPI: 4′,6-Diamidino-2-phenylindole; EGFP: enhanced green fluorescent protein; Iba1: ionized calcium-binding adapter molecule 1; IL-1β; interleukin 1 beta; IL-6: interleukin 6; LPS: lipopolysaccharide; M-sEVs: mesenchymal stem cell–small extracellular vesicles; RT-PCR: real-time polymerase chain reaction; RPCs: retinal progenitor cells; RCS: Royal College of Surgeons; TNF-α: tumor necrosis factor α; w: weeks.

    Article Snippet: Human microglia clone 3 (HMC3) cells were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China, Cat# CL-0620, RRID: CVCL_II76) and were cultured in alpha minimum essential medium (Cat# SH30265.01, Hyclone) containing 10% FBS.

    Techniques: Transplantation Assay, Staining, Injection, Labeling, Reverse Transcription Polymerase Chain Reaction, Expressing, Real-time Polymerase Chain Reaction, Comparison, Binding Assay